cellmask deep red plasma membrane stain

TOXICITY AND MITOCHONDRIAL DELIVERY OF

Localization of TPP-modified Flav-1 which fluoresces when exposed to visible light was determined using confocal microscopy HUVECs were co-stained with Hoechst CellMask Deep Red plasma membrane stain and MitoTracker Red for visualization of the nucleus plasma membrane and mitochondria respectively Modification of Flav-1 with either C2 or C8 TPP moieties did not increase

CellMaskTM Deep Red Plasma membrane Stain

CellMask TM Deep Red Plasma membrane Stain CellMask TM : : Thermo Fisher Scientific: : C10046 Similar Protocol Intracellular Invasion and Killing Assay to Investigate the Effects of Binge Alcohol Toxicity in Murine Alveolar Macrophages Victor Jimenez Jr and Fernando P Monroy Published online: 2019-01-20 Bio-protocol() Company-protocol

CellMask Deep Red Plasma membrane Stain from

The CellMask Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins These stains may be used as a segmentation tool for HCS (high-content screening) as well as to stain cellular plasma membranes for standard fluorescence microscopy The stain survives fixation but not permeabilization so it is not suitable for

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2015/4/17 Fluorophore 1 8-ANS (BSA) 2-dodecylresorufin (lipid) 5-(and-6)-carboxy-2' 7'-dichlorofluorescein (pH 9 0) 5-carboxytetramethylrhodamine [5-TAMRA] (MeOH) 5-chloromethylfluorescein (pH 7 2) 5-FAM (pH 9 0) 5-ROX (pH 7 0) 5-TAMRA (pH 7 0) 6-carboxyrhodamine 6G (pH 7 0) 6-JOE (pH 9 0) 7-amino-4-methylcoumarin (pH 7 0) Peak Peak Center Minimum

CERTIFICATE OF ANALYSIS

Product Name CellMask™ Deep Red plasma membrane stain *5 mg/mL solution in DMSO* Appearance blue solution Lot Number 1027421 LOT DATA SPECIFICATION Absorption1 Optical Density 0 77 at 651 nm 0 77 0 06 at 651 nm 3 nm 1 Solvent: Methanol Method: Optical Density 1001x dilution Betty Wood Quality Assurance Manager 08-Sep-2011 Life Technologies Corporation on

Invitrogen™ CellMask™ Deep Red Plasma membrane

The CellMask™ Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins These stains may be used as a segmentation tool for HCS (high-content screening) as well as to

Tools for High Content Screening 07

Tools for High Content Screening and Automated Imaging Mike Janes RD Manager Cellular Imaging Analysis Molecular Probes Labeling Detection Technologies 2 The Handbook on Fluorescence Detection 3 Fluorescent Dyes Proteins Nanocrystals Fluorescent Dyes Alexa Fluor 488 • MW – ~250 –1800 Da • Emission λ: ~400–850+ nm • Ext coeff : 20 000–250 000 M-1cm-1 • Designed

US Patent for Membrane staining dyes containing

The present invention is concerned with a staining dye for staining a biological cell comprising dye molecules with a positively charged polar head group for attraction to cell membrane of the cell and for refraining the dye molecules from entering the cytoplasm and one or more hydrophobic groups for interaction with phospholipids of the cell membrane

Phosphatidic acid regulates subcellular distribution of

BODIPY TR ceramide (red) and CellMask Deep Red Plasma membrane stain (blue) were used as a marker of the golgi complex and the plasma membrane respectively DIC differential interference contrast Line profiles of RA-GEF-1 intensity along the arrows (X–Y a-b c-d e-f) are shown below The red arrows show the peak of RA-GEF-1 on the plasma membrane perinuclear region or cytoplasm in

Cell Based Assays

Cell Based Assays -( 1 – 30 1132 Interest Areas Life Science Chemicals Chromatography Industries Fisherbrand Events and Exhibitions Activate Science On-Site Exhibitions Trade Shows Business Solutions Centrifuge Guide New Lab Start-Up Program Unity Lab Services Environment / Sustainability ISO certification and Quality Management System

Detail zkazky Z201850136

CellMask™ Deep Red Plasma membrane Stain- fluorescenčn farbička farbiaca plazmatick membrnu Po excitcii emituje červen fluorescenciu Max excitcia/emisia: 649/666 nm Ekvivalent CellMask™ Deep Red Plasma membrane Stain Thermo Fisher Scientific Kat č : C10046

Cell Based Assays

Cell Based Assays -( 1 – 30 1132 Interest Areas Life Science Chemicals Chromatography Industries Fisherbrand Events and Exhibitions Activate Science On-Site Exhibitions Trade Shows Business Solutions Centrifuge Guide New Lab Start-Up Program Unity Lab Services Environment / Sustainability ISO certification and Quality Management System

VW 7KLV

resonance illumination with plasma membrane dye (CellMask™ Deep Red Plasma membrane Stain) and nucleus dye (NucRed™ Live 647 ReadyProbes™ Reagent) The fluorescence intensity is normalized to the value of initial measurement The fluorophores in subsequent scans are 64% 46% 30% and 21% of the initial value for the plasma membrane dye and 92% 89% 88% and 86% of the first scan for

Methods for Cells in the Lab

Three to four days after cells are plated and mature and healthy colonies are observed on 96- and 24-well imaging plates the cells are stained with NucBlue Live ready probe reagent (R37605 ThermoFisher) and CellMask Deep Red plasma membrane stain (C10046 ThermoFisher) to visualize DNA and plasma membrane respectively Phenol red free mTeSR1

Ioannis Zervantonakis on FPbase

about FPbase help FAQs how to cite FPbase contributing API recent activity how you can help contact tools spectra viewer microscopes FP BLAST FP collections FRET calculator explore SEARCH advanced search BROWSE protein table interactive chart lineages organisms spectra references SUBMIT submit a protein submit a spectrum sign in Ioannis Zervantonakis click and drag to zoom

CellMask™ Deep Red Plasma membrane Stain

The CellMask Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins These stains may be used as a segmentation tool for HCS (high-content screening) as well as to stain cellular plasma membranes for standard fluorescence

Invitrogen CellMask Deep Red Plasma membrane Stain

The CellMask Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins These stains may be used as a segmentation tool for HCS (high-content screening) as well as to stain cellular plasma membranes for standard fluorescence microscopy The stain survives fixation but not permeabilization so it is not suitable for

CERTIFICATE OF ANALYSIS

Product Name CellMask™ Deep Red plasma membrane stain *5 mg/mL solution in DMSO* Appearance blue solution Lot Number 1027421 LOT DATA SPECIFICATION Absorption1 Optical Density 0 77 at 651 nm 0 77 0 06 at 651 nm 3 nm 1 Solvent: Methanol Method: Optical Density 1001x dilution Betty Wood Quality Assurance Manager 08-Sep-2011 Life Technologies Corporation on

CellMask™ Green Plasma Membrane Stain

CellMask™ Green Plasma Membrane Stain is designed to deliver uniform staining of the plasma membrane across a wide variety of mammalian cell types and is slow to internalize particularly compared to traditional approaches such as DiI DiO and labeled wheat germ agglutinin CellMask™ plasma membrane stains are amphipathic molecules containing a lipophilic moiety for excellent membrane

07 09 Live Cell Imaging

Plasma Membrane Stain CellMask™ Deep Red Plasma Membrane Stain (Above) Live 293 MSR cells labeled for 1 min in complete media washed and imaged •Label maintained in PM for 30-60 minutes before internalization •Formaldehyde-fixable (not shown in this slide) but not detergent resistance •Near labeling of cells after 1-10 min

Direct demonstration of discrete Ca2+ microdomains

EGTA-AM BAPTA-AM CellMask Deep Red plasma membrane stain and Lipofectamine 2000 were from Invitrogen (Paisley UK) Restriction enzymes DNA T4 ligase and calf intestinal phosphatase (CIP) were from New England Biolabs (Ipswich MA) Oligonucleotides were from Sigma-Genosys (Haverhill UK) All other reagents were obtained from Sigma-Aldrich (Dorset UK)

Cell

The plasma membrane of NMuMG cells was stained by replacing the culture medium by cell culture medium containing 5μg/mL CellMask™ Deep Red Plasma Membrane Stain (Life Technologies Darmstadt Germany) After an incubation time of 5 min at 37C the staining solution was again by normal cell replaced culture medium

Invitrogen™ Molecular Probes™ CellMask™ Deep Red

Invitrogen™ Molecular Probes™ CellMask™ Deep Red Plasmamembranfrbung Vermeidet Zelltypunterschiede die von Lektinen ausgestellt werden Marke: Invitrogen™ C10046 Weitere Details : Netto-Gewicht : 0 00450kg Artikelnummer 10734084 245 00 € / 1 Stck EU Lager 17 Bitte melden Sie sich an um diesen Artikel zu kaufen Sie bentigen ein Web-Konto? Registrieren Sie sich noch heute

Endocytic Pathways and Intracellular Transport of

Endocytic Pathways and Intracellular Transport of Aptamer-drug Conjugates in Live Cells Monitored by Single-particle Tracking Cheng Lv1 Cy3-sgc8-5FU (red) entered MCF-7 cells together with CellMaskTM Deep Red plasma membrane stain labeled plasma membrane formed vesicle (green) MCF-7 cells were seeded in a 35 mm glass bottom dish and then labeled with CellMaskTM Deep Red plasma membrane

Elucidation of Exosome Migration across the Blood

In order to image the time course of exosome upTongWei in BMECs 24 hours prior to imaging Deep Red Plasma Membrane Stain CellMask (Life Technologies) was used to label BMECs according to the manufacture protocol Briefly BMECs were incubated with CellMask in PBS for 5–10 minutes at 37 C and then washed three times PKH67-labeled exosomes (20 g) or the control PBS stained with

File:Feasibility

The cells were pre-incubated with Cellmask Deep Red Plasma Membrane Stain for 5 minutes in cell culture medium and then incubated with coated spherical MSNs at 20 μg/ml in live cell imaging medium for 1 hour while being imaged Date: 22 August 2016: Source: S1 Video from Baghirov H Karaman D Viitala T Duchanoy A Lou Y Mamaeva V Pryazhnikov E Khiroug L de Lange Davies C Sahlgren C

Cellmask Deep Red Plasma Membrane Stain

To stain the NET 167 nM SYTOX Green nucleic acid stain (Life Technologies Carlsbad CA USA) was applied for 30 min while the fungal and the neutrophil cells were stained with 5 g/mL Calcofluor white (Cyanamid NJ USA) for 15 min and a 0 5-fold solution of the CellMask Deep Red Plasma Membrane Stain (Thermo Scientific Waltham MA USA) for 15 min respectively Between the staining

Invitrogen™ CellMask™ Deep Red Plasma membrane

The CellMask Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins These stains may be used as a segmentation tool for HCS (high-content screening) as well as to stain cellular plasma membranes for standard fluorescence microscopy The stain survives fixation but not permeabilization so it is not suitable for

cellmask64 plasma membrane stains__

Deep Red plasma membrane CellMask? Plasma Membrane Stains 3 stain (Figure 2) Note: If you used suspension cells spotted on poly D-Lysine coated coverslips for the staining protocol the background may be high but the signal-to-noise ratio is still very good Figure 2 293 MSR cells labeled with CellMask? Orange plasma membrane stain using the

Invitrogen™ CellMask™ Deep Red Plasma membrane

The CellMask Plasma Membrane Stains allow fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins These stains may be used as a segmentation tool for HCS (high-content screening) as well as to stain cellular plasma membranes for standard fluorescence microscopy The stain survives fixation but not permeabilization so it is not suitable for

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